Practical Aspects of Molecular Biology and Biotechnology

From Color Change to Single Molecules: The Evolution of Enzyme-Based Diagnostics

Fundamental principles that make ELISA so effective and examine the various formats that have been adapted for different diagnostic needs.

Aneknowledge January 8, 2026

Genetic Engineering Molecular Biology Notebook

The Secret of the Blue and White Colonies: An Introduction to Blue-White Screening

Blue-white screening, a foundational molecular biology technique used to identify bacterial colonies that have successfully integrated foreign DNA.

Aneknowledge January 5, 2026

Biochemistry Genetic Engineering Microbiology

Factors affecting migration of Nucleic Acids in Agarose Gel Electrophoresis

Agarose gel electrophoresis is a molecular biology technique that can be used to separate DNA, RNA, or proteins.

Mitesh Shrestha June 25, 2024

Molecular Biology Practical Aspects of Molecular Biology and Biotechnology

Calculation for PCR Composition

The calculation is for a general conventional PCR to help beginnners.

Mitesh Shrestha June 7, 2024

Genetic Engineering Microbiology Molecular Biology

How to reduce the number of screenings for transformed bacterial colonies?

Designing experiment with controls for transformation of bacteria.

Mitesh Shrestha March 14, 2024

Genetic Engineering Microbiology Molecular Biology

Underlying Principle of Different Solutions Used for Isolation of Plasmid from Bacteria by Alkaline Lysis Method

Alkaline Lysis method is one of the most common methods for extracting plasmid from bacteria. The method was originally developed by Birnboim and Dolly in 1979.

Mitesh Shrestha February 18, 2024

Genetic Engineering Molecular Biology Practical Aspects of Molecular Biology and Biotechnology

Manual DNA Extraction Method from Human Blood

Manual DNA extraction method from human blood without using kit.

Mitesh Shrestha January 7, 2024

Genetic Engineering Microbiology Molecular Biology

Manual Technique for Extraction of DNA from Bacteria

Standard Operating Procedure for manual method of bacterial DNA extraction.

Mitesh Shrestha January 6, 2024

Genetic Engineering Microbiology Molecular Biology

Plasmid DNA Extraction from Bacteria by Alkaline Lysis Method

Plasmid DNA extraction by alkaline lysis method.

Mitesh Shrestha January 2, 2024