Calculation for PCR Composition
The calculation is for a general conventional PCR to help beginnners.
How to reduce the number of screenings for transformed bacterial colonies?
Designing experiment with controls for transformation of bacteria.
Posted inMolecular Biology
Genome Replication: Topological Problems
The most obvious problem during DNA replication is the possibility of daughter molecules getting entangled, hence, the topological problems of DNA replication.
Underlying Principle of Different Solutions Used for Isolation of Plasmid from Bacteria by Alkaline Lysis Method
Alkaline Lysis method is one of the most common methods for extracting plasmid from bacteria. The method was originally developed by Birnboim and Dolly in 1979.
CRISPR/CAS-9: HOW IT WORKS
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) is a nucleotide repetitive sequence followed by short spacer segments integrated upon exposure to invading pathogens in the genome of prokaryotes.
Development of a Highly Efficient Cancer Treatment Strategy using the Vibrionic-Driven Action of Synthetic Aminocyanines
Necrosis of cancer cells has been achieved through collaborative research between researchers at Texas A&M University, The University of Texas MD Anderson Cancer Center, and Rice University using near-infrared light to induce vibrionic modes in the aminocyanines bound to the cell membrane.
Posted inGenetic Engineering Molecular Biology Practical Aspects of Molecular Biology and Biotechnology
Manual DNA Extraction Method from Human Blood
Manual DNA extraction method from human blood without using kit.
Manual Technique for Extraction of DNA from Bacteria
Standard Operating Procedure for manual method of bacterial DNA extraction.
Posted inBiochemistry Bioinformatics Emerging Science
New Class of Antibiotics with a Novel Mode of Inhibition against World Health Organization’s Critical Listed Bacteria, Carbapenem-Resistant Acinetobacter baumannii
A new class of macrocyclic peptide-based antibiotics capable of disrupting the transport of bacterial LPS from the inner membrane to the outer surface moving through the periplasmic space and outer membrane.
Plasmid DNA Extraction from Bacteria by Alkaline Lysis Method
Plasmid DNA extraction by alkaline lysis method.