Researchers from Shenyang Pharmaceutical University and collaborating institutions have developed a single-walled carbon nanotube (SWCNT)-integrated colloidal gold immunochromatographic (CGIC) assay to rapidly detect Helicobacter pylori (H. pylori) in food samples. This pathogen, responsible for gastric diseases and linked to contaminated food and water, poses a major global health concern.
The newly developed SWCNT/CGIC test strip utilizes monoclonal antibodies targeting the H. pylori GP1 antigen. By combining colloidal gold nanoparticles with single-walled carbon nanotubes, the system significantly enhances signal intensity and detection sensitivity compared to traditional lateral-flow immunoassays.
Validation against real-time PCR revealed that the SWCNT/CGIC assay achieved a detection limit of 1 × 10³ copies/mL, with no cross-reactivity against other common foodborne microbes. It delivered results in under 15 minutes, a dramatic improvement over PCR (4–6 hours) and culture methods (3–5 days). The strips remained stable for up to 18 months at room temperature and performed effectively in detecting H. pylori in artificially contaminated foods such as milk, vegetables, and meat.
The study concludes that this nanotechnology-enhanced biosensor provides a rapid, sensitive, and low-cost diagnostic platform ideal for field applications and routine food safety checks—especially in resource-limited settings.
Reference
Song, M., Zhang, X., Song, Q., Li, S., & Xu, W. (2025). Single-Walled Carbon Nanotube-Integrated Immunochromatographic Assay for Rapid and Specific Detection of Helicobacter pylori in Food. Food Analytical Methods. https://doi.org/10.1007/s12161-025-02905-9
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