Features Comparison between Streptomyces coelicolor and Escherichia coli
Streptomyces coelicolor and Escherichia coli are two well-studied bacterial species that differ significantly in their biology, lifestyle, and applications.
Comparison between Horizontal Gene Transfer Mechanisms in Bacteria: Transformation, Conjugation and Transduction
Transformation, Conjugation, and Transduction are three main methods of horizontal gene transfer (HGT) in bacteria. These processes enable bacteria to acquire genetic material from their environment or other bacteria, contributing…
Posted inBiochemistry Genetic Engineering Microbiology
Factors affecting migration of Nucleic Acids in Agarose Gel Electrophoresis
Agarose gel electrophoresis is a molecular biology technique that can be used to separate DNA, RNA, or proteins.
How to reduce the number of screenings for transformed bacterial colonies?
Designing experiment with controls for transformation of bacteria.
Underlying Principle of Different Solutions Used for Isolation of Plasmid from Bacteria by Alkaline Lysis Method
Alkaline Lysis method is one of the most common methods for extracting plasmid from bacteria. The method was originally developed by Birnboim and Dolly in 1979.
CRISPR/CAS-9: HOW IT WORKS
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) is a nucleotide repetitive sequence followed by short spacer segments integrated upon exposure to invading pathogens in the genome of prokaryotes.
Posted inGenetic Engineering Molecular Biology
Manual DNA Extraction Method from Human Blood
Manual DNA extraction method from human blood without using kit.
Manual Technique for Extraction of DNA from Bacteria
Standard Operating Procedure for manual method of bacterial DNA extraction.
Posted inBiochemistry Bioinformatics Emerging Science
New Class of Antibiotics with a Novel Mode of Inhibition against World Health Organization’s Critical Listed Bacteria, Carbapenem-Resistant Acinetobacter baumannii
A new class of macrocyclic peptide-based antibiotics capable of disrupting the transport of bacterial LPS from the inner membrane to the outer surface moving through the periplasmic space and outer membrane.
Plasmid DNA Extraction from Bacteria by Alkaline Lysis Method
Plasmid DNA extraction by alkaline lysis method.